Monitoring autophagic flux by an improved tandem fluorescent-tagged LC3 ...
Monitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on …
Premo Autophagy Assays with Sensor LC3B, p62, or Tandem Sensor RFP-GFP …
Premo Autophagy assay kits offer the selectivity of Premo Autophagy sensors, which are based on chimeras of autophagy-associated proteins LC3B or p62, and green (GFP) and/or red …
Monitoring and Measuring Autophagy - PMC - PubMed Central …
Taking advantage of the differential sensitivity to lysosomal acidity, mRFP-GFP-LC3 tandem fluorescent-tagged LC3 can be used to visualize transition from neutral autophagosomes to …
Fluorescent Biosensors for Measuring Autophagic Flux
LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic …
Fluorescent Protein-Based Autophagy Biosensors - PMC
GFP-LC3-RFP-LC3∆G is composed of GFP-LC3 and RFP-LC3∆G linked by a substrate for the autophagy initiating protease Atg4 (Figure 3b). When autophagy is induced, Atg4 can cleave …
Monitoring Autophagy Flux and Activity: Principles and …
2020年8月26日 · Monitoring GFP-LC3 and RFP-GFP-LC3 images traces the dynamic membrane transition from autophagosome to autolysosome. Meanwhile, a fluorescent protein, Keima, has …
2015年3月9日 · LC3B‑GFP (Cat. no. P36235) or LC3B‑RFP (Cat. no. P36236) are useful for monitoring autophagosome formation, especially when combined with other FP chimeras or …
Monitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on …
Transgenic expression of a ratiometric autophagy probe …
2018年10月26日 · Here, we describe a novel autophagy reporter mouse (TRGL6) stably expressing a dual-fluorescence-tagged LC3 (tfLC3, mRFP-eGFP-LC3) by transgenesis …
Quantitative determination of autophagy flux by probes
2021年1月1日 · When using GFP-mRFP-LC3 fusion protein for cell experiments, observing the intensity changes of red fluorescence and green fluorescence at the same time can accurately …