Lipofectamine™ 3000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
Lipofectamine - Wikipedia
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid …
Lipofectamine 2000 | Thermo Fisher Scientific - US
Invitrogen™ Lipofectamine™ 2000 Transfection Reagent is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats (e.g. 96-well).
Lipofectamine™ 2000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Lipofectamine 3000 Transfection Reagent:
l transfection varies. Start any new transfection by testing the recommended two concentrations of LipofectamineTM 3000 Reagent to deter. om/termsandconditions. If you have any questions, please contact Life Technologies at www.lifete. ed Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limi.
Transfect plasmid DNA and siRNA at the same time using Lipofectamine® 2000 Reagent by adding 30 pmol (~0.6 μg) of siRNA per 1 μg of DNA. mRNA can be transfected in a 24-well plate using Lipofectamine® 2000 Reagent by adding 0.5–1 μg of mRNA per well. Transfect cells according to the following chart. Volumes are given on a per-well basis.
Molecular mechanism of Lipofectamine 3000 - Labster
Lipid-based transfection is based on the electrostatic interaction between DNA-transfection reagent complexes and the cell membrane. Here, reagent P3000 plays a crucial role. After this interaction, complexes enter the cell via endocytosis, leading to the formation of an endosome.
Lipofectamine® Reagent for the transfection of DNA into eukaryotic …
Lipofectamine™ Reagent is suitable for the transfection of DNA into eukaryotic cells (1), and is a 3:1 (w/w) liposome formulation of the polycationic lipid.
InvitrogenTM LipofectamineTM 3000 Transfection Reagent, a reagent developed to improve delivery and enable use of new technologies, can be used in more relevant systems and enables faster and more reliable outcomes.