How to explain "negative/white" Bands on a Western Blot?
I am trying to detect STAT3, pstat3, and beta-catenin expression in my rat spinal cord tissue lysate. However, whenever I run western blot I am getting white bands.
What are the white bands on western blot - ResearchGate
I was trying to do western blot for Erythropoietin, as you can see the procedure was unsuccesful but there are some unexpected white bands on the blots. Can anyone tell me what might be causing...
Western Blot Doctor™ — Protein Band Appearance Problems
The Western Blot Doctor is a self-help guide that enables you to troubleshoot your western blotting problems. In this section, you can find solutions to issues related to protein band appearance. Other sections in the Western Blot Doctor: Band Appearance Problems; Blot Background Problems; Signal Strength Problems; Band Size and Pattern Problems
Troubleshooting tips for western blot - Abcam
Explore our troubleshooting tips from experts on western blot issues like no signal, high background, multiple bands, and more.
Western Blot Troubleshooting White Bands | Sino Biological
But why are there white bands in a Western Blot / WB? The following Western Blot troubleshooting guide is supposed to give you possible causes and corresponding solutions and assist you get reliable results in your western blotting.
White bands surrounded by black (ghost bands) are caused by an intense localized signal that completely exhausts the ECL reaction with a quick burst of light. Therefore there is no light produced during development and a white band occurs. Use less primary, secondary, or protein. Use less total protein loaded into each lane. You may perform an
Western Blot Doctor™ — Blot Background Problems - Bio-Rad
White spots or white areas on the blot may be due to trapped air bubbles or unwetted membrane areas where protein will not bind. Both can be prevented with care. Possible causes:
Troubleshooting Western Blots with the Western Blot Doctor™
Free guide helps troubleshoot Western blot problems, from band appearance to signal strength. Includes tips, techniques, and product selector tool.
Western Blot Troubleshooting Guide - R&D Systems
A comprehensive Western Blot troubleshooting guide for solutions to common issues such as no bands, faint bands, extra bands, high background, and more.
Western blot troubleshooting guide! - Jackson Immuno
Apr 27, 2017 · Nitrocellulose may have less auto – fluorescence than PVDF when conducting a fluorescent Western blot. Is the ECL blot in negative – black membrane with white bands? Antibody or antigen concentration is too high. Titrate antibody and sample to optimize. Observe unstained membrane in Western blot detection instrument to confirm autofluorescence.