2021年5月22日 · The indirect ELISA is used for the quantitative estimation of antibodies in the serum and other body fluids. In this method, specimens are added to microtiter plate wells coated with antigen to which specific antibodies are to be detected.

2023年4月23日 · In HIV testing, a blood or saliva specimen is collected for testing, typically using indirect ELISA-based tests. The ELISA is a screening tool for HIV detection, but not diagnostic. Diagnosis requires further testing by Western blot due to potential false positives.

2024年4月10日 · Indirect ELISA, short for Enzyme-Linked Immunosorbent Assay, is a widely used technique in immunology and diagnostic laboratories for the detection and quantification of antibodies in various biological samples, such as serum or other body fluids.

An indirect ELISA is similar to a direct ELISA in that an antigen is immobilized on a plate, but it includes an additional amplification detection step. First, an unconjugated primary detection antibody is added and binds to the specific antigen.

This chapter provides the procedures required for carrying out indirect ELISA, one of the many forms of ELISA, to detect polystyrene-immobilized antigen. Methodological approaches to optimize this assay technique are also described, a prerequisite for automation and multiplexing.

2022年5月17日 · Enzyme-Linked Immunosorbent Assay (ELISA) is a modern molecular technique for the detection of antigen-antibody interaction with the help of an enzyme. It is one of the sensitive enzyme immunoassay techniques for the detection of the presence of antigen or antibody and quantification as well in the case of clinical diagnosis of many diseases.

It can precede the development of a sandwich enzyme-linked immunosorbent assay (ELISA) in which optimal antibody concentrations are applied for the quantitative measurement of the antigen.

Discover the steps of the indirect ELISA protocol with Leinco for precise antibody detection in your research.

2025年1月25日 · In an indirect ELISA, we quantify antigen-specific antibody rather than antigen. We can use indirect ELISA to detect antibodies against many types of pathogens, including Borrelia burgdorferi (Lyme disease) and HIV. There are three important differences between indirect and direct ELISAs as shown in Figure \(\PageIndex{4}\). Rather than using ...

2020年3月13日 · What is an Indirect ELISA? How is it different from the Direct ELISA? An enyzme-linked immunosorbent assay (ELISA) can be done with a varitey of modifications to its basic protocol. For example, the direct ELISA uses an enzyme-labeled primary antibody conjugate to detect immobilized antigens.