5 days ago · Functional analysis methods are crucial for assessing enzyme performance and catalytic properties. Laccase, a well-known blue multi-copper oxidase, holds immense potential in diverse industries such as pharmaceuticals, paper and pulp, food and beverages, textiles, and biorefineries due to its clean oxidation process and versatility in handling ...

Jan 20, 2025 · Amide synthetase activity assay. All high-throughput assays (hot spot screen, iterative site saturation mutagenesis, substrate scope, ML predictions validation, and ML prediction exploration) were ...

Jan 7, 2025 · In this study, we introduced the detectEV assay as a new, simple, highly sensitive and precise functional test, able to define EV integrity and identity, by evaluating the EV-associated enzymatic activity, using a FDA-based enzymatic method (Adam & Duncan, 2001; Sırt Çıplak & Akoğlu, 2020). Throughout this assay, we considered various ...

Jan 25, 2025 · Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation.

2 days ago · To assess the enzymatic activity of wild-type and mutant PI4KA protein fragments, we purified both the wild type and p.Ala940Val mutant form of PI4KA enzyme, as shown in Fig. 4C, and conducted enzyme activity assays with the ptt5 plasmid serving as the negative control.

1 day ago · Since the enzymatic-like activity of Fe 3 O 4 was reported, research on iron-based nanozymes has undergone vigorous development. However, most of previously reported iron-based nanozymes, including iron single-atom nanozymes, always rely on free reactive oxygen species (ROS) to exert their catalytic effects, especially the OH derived from Fenton-like …

Jan 13, 2025 · By improving the activity assessment of PET-degrading enzymes, their performance-based comparison and selection are also facilitated, which enables precise selection of the optimal biocatalyst...

Jan 10, 2025 · By employing this holistic and targeted assay engineering strategy, a deeper understanding of the factors affecting BSH activity was achieved, leading to a significant enhancement in enzymatic performance, with a 1.45- and 0.87-fold increase in BSH activity from H. coagulans ATCC 7050 and L. plantarum ATCC 10012, respectively, at 37 °C.

Jan 15, 2025 · Enzymatic activity assay. Fluorescence resonance energy transfer assay was performed to determined NEP and ECE activity using black 96-well plates . Mice cerebral cortex or hippocampus from APP/PS1 and age-matched WT mice at 3, 6, 9, 12 month were homogenized in ice cold phosphate-buffered saline (0.1 M PBS, pH 7.4) containing protease ...

Jan 11, 2025 · By combining modifications to the activity, we developed an indicator dynamic squeezing index (DSI) coupled with the active center to improve the activity of enzymes with varying degrees of...