The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

Promega offers the pGL4 series of firefly and Renilla luciferase vectors designed for use with the DLRTM Assay Systems. These vectors may be used to cotransfect mammalian cells with experimental and control reporter genes.

Promega offers the pGL4 series of firefly and Renilla luciferase vectors designed for use with the DLR Assay Systems. These vectors may be used to co-transfect mammalian cells with experimental and control reporter genes.

The dual luciferase assay is performed by sequentially measuring the firefly and Renilla luciferase activities of the same sample, with the results expressed as the ratio of firefly to Renilla luciferase activity (Fluc/Rluc).

The dual luciferase assay is performed by sequentially measuring the firefly and Renilla luciferase activities of the same sample, with the results expressed as the ratio of firefly to Renilla luciferase activity (Fluc/Rluc).

2013年1月24日 · The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Based on this observation, we developed a high sensitive dual secreted luciferase assay which allows the determination of both activities in a single cotransfection experiment. We demonstrate the versatility and robustness of the assay for the normalization of reporter gene activities.

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are …

2022年7月15日 · A Dual Luciferase Assay allows the measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. It is also called a Bioluminescent Assay. Firefly luciferase is a very sensitive genetic reporter.

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.

Dual-spectral luciferase assays are ideal for performing time-course assays using a secreted luciferase to measure the experimental promoter activity and an intracellular luciferase under the control of a housekeeping gene promoter to measure cell viability.

Some important features of luciferase assays (Brasier & Ron, 1992): This article provides information about the luciferase assay, including the process and materials you will need to prepare before you perform the assays. What is a Luciferase Reporter Assay?

We accomplish this using a high-throughput, dual-luciferase, in vitro reporter screen to deconstruct alpha-synuclein expression in 293T cells. The alpha-synuclein promoter is first cloned into a reporter plasmid containing the firefly luciferase gene.

Luciferases are commonly incorporated into genetic reporter systems for evaluation of eukaryotic gene expression. Often a combination of two luciferase variants with differing substrate afinities are used in a dual-luciferase assay format.

The Firefly/Renilla Dual Luciferase Assay allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase ...

The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.

2024年3月21日 · In this study, we explored the potential of using a green bioluminescence-emitting click beetle luciferase, CBG99, and a red bioluminescence-emitting firefly luciferase mutant, Akaluc, together to simultaneously monitor two cell populations.

2025年1月13日 · In this dual-luciferase reporter system, PpyLuc serves as the primary reporter for detecting miRNA interactions, while RLuc functions as a control reporter for normalization [[45], [46], [47]]. Therefore, in this investigation, the pmirGLO plasmid was modified by replacing the original Ppy Luc encoding sequence with that of Ppe 146-LGR to ...

2024年12月30日 · Then MUT plasmids (MUT-circ-PDE1C, MUT-SGTB 3’UTR) were used as control groups. The monolayer CHON-001 cells in the 48-well plates were transfected with luciferase plasmid and mimic NC or miR-766-3p mimic. Luciferase activity determination by Dual-luciferase Reporter Detection Kit (Promega) was performed after 48 h. RIP assay

2025年1月13日 · P, Q The luciferase activity of the SLC7A11 promoter in the F-Luc/R-Luc system was measured using the Dual-Lumi™ II Luciferase Assay Kit. Data are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significant, by Student’s t test between two groups and by one-way ANOVA followed by ...

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are …

Promega offers a choice of 3 different luciferase reporters: NanoLuc® Luciferase (Nluc,19kDa), Renilla Luciferase (Rluc, 36kDa) and Firefly Luciferase (Fluc, 61kDa) which vary in size, brightness, and protein half-life. Luc/Luc+: Original luciferase gene used in older luciferase reporter systems.

The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells containing genes for firefly and Renilla luciferases, grown in 96- or 384-well plates. The Dual-Glo® Luciferase Reagent can be added directly to cells in growth medium without washing or preconditioning.