Calcium flux assays are widely used for in-cell measurement of agonist-stimulated and antagonist-inhibited signaling through G protein–coupled receptors (GPCRs), a large and active target class relevant in drug discovery.

2021年7月22日 · A calcium flux assay is a fluorescence-based assay used to detect intracellular calcium mobilization in cells and follow the release of Ca 2+ into the cytoplasm. It involves preloading cells with a calciumsensitive fluorescent dye that is …

Calcium ions play an especially critical role in cellular signaling. As a signaling messenger, calcium is involved in everything from muscle contractions, to cell motility, to enzyme activity. Cells tightly regulate calcium to ensure that the cytoplasmic concentration of Ca2+ …

Calcium flux assays are fluorescence-based assays designed to detect the transient release of intracellular calcium stores. These assays typically rely on cell-permeable dyes with fluorescence properties that are calcium sensitive.

2020年7月3日 · Calcium flux assays detect intracellular calcium mobilisation in cells and follow the release of Ca 2+ into the cytoplasm 6.

Run untreated cells to establish baseline; run “positive” control to establish maximum Ca++ flux ratio; run EGTA inhibited cells to approximate low (minimal) response.

Fluorescent calcium indicators and dyes to measure Ca+ flux used in calcium imaging experiments. Calcium ions (Ca2+) play vital cellular physiology roles in signal transduction pathways, in neurotransmitter release, in contraction of all muscle cell types, as enzyme cofactors, and in fertilization.

Calbryte™-520 is exceptionally bright upon binding to Ca 2+ with very high signal to noise ratio when compared to other existing calcium indicators (such as Fluo-3 AM, Fluo-4 AM and Rhod-2 AM). Calbryte™-520 AM also have improved intracellular retention and loading efficiency.

The following protocol, our most widely-used method for intracellular calcium flux determination, employs the probe Indo-1. Indo-1 requires UV (350nm) excitation, and has a dual emission pattern: dye bound to free intracellular calcium will emit at …

The principle of flow cytometric Ca 2+ flux measurement is based on changes in fluorescence intensity or emission wavelength of a fluorophore following the chelation of calcium ions. This is commonly reported as a plot of fluorescence intensity against time.