Laccase Functional Analysis: Substrates, Activity Assays, Challenges ...
5 天之前 · Functional analysis methods are crucial for assessing enzyme performance and catalytic properties. Laccase, a well-known blue multi-copper oxidase, holds immense …
仅显示来自 chemistry-europe.onlinelibrary.wiley.com 的搜索结果DetectEV: A functional enzymatic assay to assess integrity and ...
In this study, we introduced the detectEV assay as a new, simple, highly sensitive and precise functional test, able to define EV integrity and identi…
Accelerated enzyme engineering by machine-learning guided cell …
2025年1月20日 · Amide synthetase activity assay. All high-throughput assays (hot spot screen, iterative site saturation mutagenesis, substrate scope, ML predictions validation, and ML …
DetectEV: A functional enzymatic assay to assess integrity and ...
2025年1月7日 · In this study, we introduced the detectEV assay as a new, simple, highly sensitive and precise functional test, able to define EV integrity and identity, by evaluating the EV …
Fine tuning enzyme activity assays for monitoring the enzymatic ...
2025年1月25日 · Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the …
Identifying novel heterozygous PI4KA variants in fetal …
2 天之前 · To assess the enzymatic activity of wild-type and mutant PI4KA protein fragments, we purified both the wild type and p.Ala940Val mutant form of PI4KA enzyme, as shown in Fig. 4C, and conducted enzyme activity assays with the …
Free reactive oxygen species-independent dual enzymatic activity …
1 天前 · Since the enzymatic-like activity of Fe 3 O 4 was reported, research on iron-based nanozymes has undergone vigorous development. However, most of previously reported iron …
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Fine tuning enzyme activity assays for monitoring the enzymatic ...
2025年1月13日 · By improving the activity assessment of PET-degrading enzymes, their performance-based comparison and selection are also facilitated, which enables precise …
Targeted Assay Engineering Enhances Bile Salt Hydrolase Activity in
2025年1月10日 · By employing this holistic and targeted assay engineering strategy, a deeper understanding of the factors affecting BSH activity was achieved, leading to a significant …
Dynamic alteration of neprilysin and endothelin-converting …
2025年1月15日 · Enzymatic activity assay. Fluorescence resonance energy transfer assay was performed to determined NEP and ECE activity using black 96-well plates . Mice cerebral …
Tailoring industrial enzymes for thermostability and activity …
2025年1月11日 · By combining modifications to the activity, we developed an indicator dynamic squeezing index (DSI) coupled with the active center to improve the activity of enzymes with …
