Two-photon excitation (TPE) microscopy enables imaging of living tissues up to a depth of one millimeter, which is 6 to 10-fold deeper than with confocal microscopy. Like confocal microscopy, TPE ...

Some approaches to enhance temporal resolution of laser scanning microscopes include optical methods of multiplexing the excitation beam, the design of spinning disks for two-photon excitation 27 ...

Before uncaging, cellular fluorescence was negligible; during continuous two-photon excitation at 790 nm, we detected no photolysis, but two-photon uncaging at 730 nm selectively activated HCCC2 ...

a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence excitation only with the diffraction limited spot of the objective lens, thus creating an inherent ...