Here, we present an approach called Integral-Omics, which enables sequential extraction of metabolites, lipids, genomic DNA, total RNA, proteins, and phosphopeptides from a single biopsy-level tissue ...

Tissue processing advance can label proteins at the level of individual cells across whole, intact rodent brains and other large samples just as fast and uniformly as in dissociated single cells.

A new technology developed at MIT enables scientists to label proteins across millions of individual cells in fully intact 3D tissues with unprecedented speed, uniformity, and versatility. Using the ...

To evaluate the outcome of miRNA extraction from mouse brain tissues, the standard UV absorption and PAGE analysis were used. The UV measurements have shown the extracted RNA samples were free from ...

but methods are needed to track and quantify subcellular protein movement within tissue environments. Here we present a semiautomated approach to quantify subcellular protein location for hundreds of ...