A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

Sanger sequencing is the unsung workhorse of molecular biology, one whose value has long been undermined by the tedious nature of processing its data. Converting files of raw sequencing data into ...

Data will be returned within two working days; a premium service can be arranged with quicker turnaround ... The effects of template concentration on sequence quality can be seen in our technote: ...

Large templates (cosmids, BACs, PACs) We require 500 ng – 1µg of template DNA for reaction. Primers We have several standard primers for the most common vectors and for bacterial 16S rDNA sequencing.

In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.