A reusable heat blanket with a window cutout for viewing cultures is provided for maintaining temperature in the culture vessel ... Each of the batch runs lasted 7 days. Daily off-line ...

Here we used a 7.5-liter vessel and operated the system in fed-batch mode. We added a Mettler ... at 88% at 72 hours elapsed fermentation time. Culture broth pH was well maintained at 5.0. We ...